Saturday, January 12, 2008

Question about signal cleavage site of SDHA flavoprotein:

N-term sequencing of bovine FP gave SAKVSDA :
Birch-Machin, M. A., Farnsworth, L., Ackrell, B. A., Cochran, B., Jackson, S.,Bindoff, L. A., Aitken, A., Diamond, A. G. & Turnbull, D. M. (1992). The sequence of the flavoprotein subunit of bovine heart succinate dehydrogenase.
J Biol Chem 267, 11553-8.

However for the porcine FP the N-term sequence is SSAKV:
Huo, X., Su, D., Wang, A., Zhai, Y., Xu, J., Li, X., Bartlam, M., Sun, F.
& Rao, Z. (2007). Preliminary molecular characterization and crystallization of mitochondrial respiratory complex II from porcine heart. Febs J 274, 1524-9.

(The latter cutting point was confirmed for human FP by mass spec,
but I don't remember the reference.)

Wednesday, January 2, 2008

Recent Article - also on dispensibility of heme

Oyedotun KS, Sit CS, Lemire BD. The Saccharomyces cerevisiae succinate dehydrogenase does not require heme for ubiquinone reduction.
Biochim Biophys Acta. 2007 Dec;1767(12):1436-45.
Epub 2007 Sep 29.