Sunday, October 18, 2015

What happened to the Complex II page on the Ed Berry site at LBNL (sb20.lbl.gov)?

The site at LBNL is probably down for good now, but the pages are being moved to a new site with the help of digitalocean and noip: http://elcamino.ddns.net/SQR
The complex III (cyt bc1) site is also there: http://elcamino.ddns.net/cytbc1/bc1project
and the old Berkeley Berry Group page: http://elcamino.ddns.net/cytbc1/berrygroup.html

Tuesday, May 27, 2008

Isoforms of C-II subunits in A. suum

Iwata F, Shinjyo N, Amino H, Sakamoto K, Islam MK, Tsuji N, Kita K.

Change of subunit composition of mitochondrial complex II (succinate-ubiquinone reductase/quinol-fumarate reductase) in Ascaris suum during the migration in the experimental host.
Parasitol Int. 2008 Mar;57(1):54-61. Epub 2007 Aug 25.

Monday, May 26, 2008

New Article: FRD mutant affects dicarb binding, CAP is open!

Thomas M. Tomasiak, Elena Maklashina, Gary Cecchini, and Tina M. Iverson-

A Threonine on the Active Site Loop Controls Transition State Formation in Escherichia coli Respiratory Complex II*

J. Biol. Chem., Vol. 283, 15460-15468, May 30, 2008

Saturday, April 19, 2008

Role of complex II in regulating ROS production by respiratory chain

Title: High rates of superoxide production in skeletal-muscle mitochondria respiring on both complex I- and complex II-linked substrates
Muller FL, Liu YH, Abdul-Ghani MA, et al.
Source: BIOCHEMICAL JOURNAL Volume: 409 Pages: 491-499 Part: Part 2 Published: JAN 15 2008

Methods paper-

Methods Mol Biol. 2008;432:65-81.

Click here to read
Preparation of Respiratory Chain Complexes from Saccharomyces cerevisiae Wild-Type and Mutant Mitochondria : Activity Measurement and Subunit Composition Analysis.

Lemaire C, Dujardin G.

CNRS, Centre de Génétique Moléculaire, Gif sur Yvette, France.

Monday, February 18, 2008

Complex II andthe Hypoxic Response

Robert D. Guzy, Bhumika Sharma, Eric Bell, Navdeep S. Chandel, and Paul T. Schumacker

Loss of the SdhB, but Not the SdhA, Subunit of Complex II Triggers Reactive Oxygen Species-Dependent Hypoxia-Inducible Factor Activation and Tumorigenesis

Molecular and Cellular Biology, January 2008, p. 718-731, Vol. 28, No. 2

Saturday, January 12, 2008

Question about signal cleavage site of SDHA flavoprotein:

N-term sequencing of bovine FP gave SAKVSDA :
Birch-Machin, M. A., Farnsworth, L., Ackrell, B. A., Cochran, B., Jackson, S.,Bindoff, L. A., Aitken, A., Diamond, A. G. & Turnbull, D. M. (1992). The sequence of the flavoprotein subunit of bovine heart succinate dehydrogenase.
J Biol Chem 267, 11553-8.

However for the porcine FP the N-term sequence is SSAKV:
Huo, X., Su, D., Wang, A., Zhai, Y., Xu, J., Li, X., Bartlam, M., Sun, F.
& Rao, Z. (2007). Preliminary molecular characterization and crystallization of mitochondrial respiratory complex II from porcine heart. Febs J 274, 1524-9.

(The latter cutting point was confirmed for human FP by mass spec,
but I don't remember the reference.)