What happened to the Complex II page on the Ed Berry site at LBNL (sb20.lbl.gov)?

The site at LBNL is probably down for good now, but the pages are being moved to a new site with the help of digitalocean and godaddy: https://www.cytbc1.com/SQR/
The complex III (cyt bc1) site is also there: https://www.cytbc1.com/cytbc1/
and the old Berkeley Berry Group page: https://www.cytbc1.com/cytbc1/berrygroup.html

Isoforms of C-II subunits in A. suum

Iwata F, Shinjyo N, Amino H, Sakamoto K, Islam MK, Tsuji N, Kita K.

Change of subunit composition of mitochondrial complex II (succinate-ubiquinone reductase/quinol-fumarate reductase) in Ascaris suum during the migration in the experimental host.
Parasitol Int. 2008 Mar;57(1):54-61. Epub 2007 Aug 25.

New Article: FRD mutant affects dicarb binding, CAP is open!

Thomas M. Tomasiak, Elena Maklashina, Gary Cecchini, and Tina M. Iverson-

A Threonine on the Active Site Loop Controls Transition State Formation in Escherichia coli Respiratory Complex II*

J. Biol. Chem., Vol. 283, 15460-15468, May 30, 2008

Role of complex II in regulating ROS production by respiratory chain

Title: High rates of superoxide production in skeletal-muscle mitochondria respiring on both complex I- and complex II-linked substrates
Muller FL, Liu YH, Abdul-Ghani MA, et al.
Source: BIOCHEMICAL JOURNAL Volume: 409 Pages: 491-499 Part: Part 2 Published: JAN 15 2008

Methods paper-

Methods Mol Biol. 2008;432:65-81.

Preparation of Respiratory Chain Complexes from Saccharomyces cerevisiae Wild-Type and Mutant Mitochondria : Activity Measurement and Subunit Composition Analysis.

Lemaire C, Dujardin G.

CNRS, Centre de Génétique Moléculaire, Gif sur Yvette, France.

Complex II andthe Hypoxic Response

Robert D. Guzy, Bhumika Sharma, Eric Bell, Navdeep S. Chandel, and Paul T. Schumacker

Loss of the SdhB, but Not the SdhA, Subunit of Complex II Triggers Reactive Oxygen Species-Dependent Hypoxia-Inducible Factor Activation and Tumorigenesis

Molecular and Cellular Biology, January 2008, p. 718-731, Vol. 28, No. 2

Question about signal cleavage site of SDHA flavoprotein:

N-term sequencing of bovine FP gave SAKVSDA :
Birch-Machin, M. A., Farnsworth, L., Ackrell, B. A., Cochran, B., Jackson, S.,Bindoff, L. A., Aitken, A., Diamond, A. G. & Turnbull, D. M. (1992). The sequence of the flavoprotein subunit of bovine heart succinate dehydrogenase.
J Biol Chem 267, 11553-8.

However for the porcine FP the N-term sequence is SSAKV:
Huo, X., Su, D., Wang, A., Zhai, Y., Xu, J., Li, X., Bartlam, M., Sun, F.
& Rao, Z. (2007). Preliminary molecular characterization and crystallization of mitochondrial respiratory complex II from porcine heart. Febs J 274, 1524-9.

(The latter cutting point was confirmed for human FP by mass spec,
but I don't remember the reference.)

Recent Article - also on dispensibility of heme

Oyedotun KS, Sit CS, Lemire BD. The Saccharomyces cerevisiae succinate dehydrogenase does not require heme for ubiquinone reduction.
Biochim Biophys Acta. 2007 Dec;1767(12):1436-45.
Epub 2007 Sep 29.

Heme not required for Complex II function, important for stability

Tran QM, Rothery RA, Maklashina E, Cecchini G, Weiner JH.
Escherichia coli succinate dehydrogenase variant lacking the heme b.
Proc Natl Acad Sci U S A. 2007 Nov 7; [Epub ahead of print]

Glutathionylation of CII flavoprotein reduces ROS production, lost in ischemia/reperfusion injury

Yeong-Renn Chen, Chwen-Lih Chen, Douglas R Pfeiffer, and Jay L. Zweier
Mitochondrial complex II in post-ischemic heart: Oxidative injury and the role of protein S-glutathionylation
J. Biol. Chem, 10.1074/jbc.M702294200
(Complex II was glutathionylated at C90 which is C46 in the mature sequence, 10 residues from the FAD covalent attachment site and spatially near the surface and near the adenine end of FAD. )

New Q-site article

Szeto SS, Reinke SN, Sykes BD, Lemire BD. Ubiquinone-binding site mutations in the saccharomyces cerevisiae succinate dehydrogenase generate superoxide and lead to the accumulation of succinate.
J Biol Chem. 2007 Jul 18; PMID: 17636259

Firmer evidence for involvement of Complex II in Huntingtons

Benchoua A, Trioulier Y, Zala D, Gaillard MC, Lefort N, Dufour N, Saudou F, Elalouf JM, Hirsch E, Hantraye P, Déglon N, Brouillet E.
Involvement of mitochondrial complex II defects in neuronal death produced by N-terminus fragment of mutated huntingtin.
Mol Biol Cell. 2006 Apr;17(4):1652-63.

New Article

Baysal BE, Lawrence EC, Ferrell RE.
Sequence variation in human succinate dehydrogenase genes: evidence for long-term balancing selection on SDHA.
BMC Biol. 2007 Mar 21;5:12. PMID: 17376234

New Complex II Polymorphism database:

Database address:
http://chromium.liacs.nl/lovd_sdh/
Publication:
Jean-Pierre Bayley, Peter Devilee1, and Peter EM Taschner.(2005) The SDH mutation database: an online resource for succinate dehydrogenase sequence variants involved in pheochromocytoma, paraganglioma and mitochondrial complex II deficiency
BMC Medical Genetics 2005, 6:39 doi:10.1186/1471-2350-6-39

Recent Article: Complex II in leukemia?

A Recurrent Stop-Codon Mutation in Succinate Dehydrogenase Subunit B Gene in Normal Peripheral Blood and Childhood T-Cell Acute Leukemia.
Bora E. Baysal, May 2007, PLoS ONE Online Journal

Wolinella succinogenes QFR has a cis-Asp!

(larger image) Wolinella succinogenes QFR flavoprotein has a cis-Asp! Model w atom labels is 2BS2, maps calculated with phases from it using SF's sf2bs2.cif. Other model is chicken complex II, superimposing the FAD domain. Green and red maps are positively and negatively contoured Fo-Fc maps- green on the chickenmodel shows where the atom should be, red on the Wolli atom shows it is misplaced. Apparently this cis-peptide occurs in all membrane-bound SDH/QFR enzymes solved to date. Soluble FCc FRD from Shewanella has a deletion here and no cis-peptide. The cis-peptide introduces a bulge which accomodates the extra residue. This is near the active site and an enigmatic conserved protein-bound metal ion (yellow crosses at bottom center).

Recent Article

Dicoumarol impairs mitochondrial electron transport and pyrimidine biosynthesis in human myeloid leukemia HL-60 cells. Biochem Pharmacol. 2007 Feb 1;73(3):427-39. Epub 2006 Oct 21. PMID: 17123468 (Dicoumarol stimulates ROS production, potentiated by Complex II Q-site inhibitors.)

Recent Article

Bacsi A, Woodberry M, Widger W, Papaconstantinou J, Mitra S, Peterson JW, Boldogh I. Localization of superoxide anion production to mitochondrial electron transport chain in 3-NPA-treated cells. Mitochondrion. 2006 Oct;6(5):235-44.
PMID: 17011837

For Comments:

Only moderators can initiate new posts. Anyone can add a comment to an existing post, but the comment should be related. So this entry is for general comments not related to any of the articles. Also for instructions to new users.

New Article:

Silkin Y, Oyedotun KS, Lemire BD.
The role of Sdh4p Tyr-89 in ubiquinone reduction by the Saccharomyces cerevisiae succinate dehydrogenase.
Biochim Biophys Acta. 2006 Dec 6; [Epub ahead of print]
PMID: 17208193